Plant Salt Resistance Protein Function Analysis Service

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Plant Salt Resistance Protein Function Analysis Service

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Salinity in the soil is one of the most important abiotic stresses in agriculture, severely reducing crop yields. Therefore, we provide molecular biology and genetics services to study salt resistance determinants and their regulatory mechanisms in plants. Our services provide a basis for the development of new varieties of highly salt-resistant crops.

CD BioSciences focuses on plant protein research and provides analysis services on the function of plant salt-resistant proteins, including identifying salt-resistant proteins, determining salt-resistant function in transgenic plants, and action mechanisms analysis.

Processes of salt resistance in plant cells. Figure 1. Processes of salt resistance in plant cells. (Hao S, et al., 2021)

Service Content

Salt Stress Response Protein Identification Service

We analyze the overall changes in gene expression or protein abundance under salt stress through high-throughput technologies such as

  • RNA-seq
  • Microarray
  • Mass spectrometry-based proteomics

We provide our clients with differentially expressed proteins for further functional analysis.

Transgenic Plants Salt Resistance Function Analysis Service

We offer transgenic strain acquisition services to help our clients analyze the resistance of transgenic plants to salt stress. Our plant salt resistance assay services include

  • Seed germination effect assay.
  • Morphological and structural changes in the root system assay.
  • Relative cellular water content assay.
  • Salt accumulation assay.
  • Reactive oxygen species (ROS) assay.

Plant Protein Salt Resistance Mechanism Analysis Service  

Combined with structural domain analysis, localization experiments, and interacting proteins analysis, we elucidate the pathways involved in the salt resistance regulation of target proteins for our clients.

Mechanism type Service content
Osmotic pressure regulation pathways. Assay the content of organic substances and inorganic ions.
ROS scavenging pathway. Determination of enzymatic antioxidant and non-enzymatic antioxidant.
Ion regionalization. Transmembrane potential gradient assay.
Involved in Ca2+ -dependent signaling pathway. Interaction screening and identification of proteins related to SOS pathway, ABA pathway, CDPK pathway, etc.
Involved in Ca2+ -independent signaling pathway. Interaction screening and identification of proteins related to MAPK pathway.
Regulation of salt tolerance-related gene expression. Detection of expression levels of Osmoregulation-related genes, ion transport protein-related genes, antioxidant-related genes, signal transduction-related genes, etc.
Involved in the regulation of nitrogen metabolism. Assay the asparagine and ammonium (NH4+) accumulation.
Effects on the photosynthetic system. Assay the chlorophyll and carotenoid content.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details. 

References

  1. Hao S, et al. (2021). A Review on Plant Responses to Salt Stress and Their Mechanisms of Salt Resistance. Horticulturae. 7(6):132.
  2. Roy SJ, et al. (2014). Salt resistant crop plants. Curr Opin Biotechnol. 4(26):115-124.

For research use only, not for clinical use.