Plant Protein Large-Scale Expression and Purification Service

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Plant Protein Large-Scale Expression and Purification Service

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CD BioSciences has extensive experience in recombinant protein preparation and has fermentation tanks of different capacities to meet clients' production requirements. We are also capable of large-scale protein purification and can efficiently control the purification process.

Scale Expression and Purification Service.

Proteins are the basic life and organic substances that constitute plant cells. The study of proteins is an indispensable part of biological research, and the preparation of gram-scale proteins is a prerequisite. Large-scale production and purification of proteins require technological bottlenecks and core equipment.

Service Content

Large-scale plant protein expression and purification service

Large-scale protein expression system Service details
Prokaryotic protein expression system
  • Gene optimization and plasmid construction.
  • Small-scale expression and optimization of fermentation conditions.
  • Fermentation for large-scale protein production.
  • Large-scale protein purification (homologous recombinant protein for crystallization, purity ≥ 98%; partially purified homologous recombinant protein, purity ≥ 70%).
Yeast protein expression system
  • Codon optimization and plasmid construction.
  • Positive and high copy strain screening.
  • Domestication of engineering strains.
  • Optimization of fermentation conditions.
  • Large-scale protein production and purification.
Arbovirus-insect protein expression system
  • Gene optimization.
  • Preparation of baculovirus plasmid and viral packaging, supply of purified baculovirus material with high concentration ( ≥ 109fu/ml) carrying the target gene.
  • Virus mass production.
  • Large-scale protein expression and purification (large-scale protein production up to 500L).
Mammalian cell protein expression system
  • Gene optimization and vector construction.
  • Plasmid transfection and selection of stably transfected cell lines.
  • Optimization of culture medium and fermentation conditions.
  • Bulk protein preparation and purification (up to 50L of media and cells from stable cell lines).

Other services

  • Designing rational experimental programs.
  • Selection of suitable fusion proteins.
  • Improve protein activity and stability.
  • Label removal.
  • Dual-phase culture and metabolic transformation.
  • Media optimization, based on component titration, media blending, spent media analysis, stoichiometric analysis, rational culture media design, and systematic approaches.

Application Fields

  • Medical-pharmaceutical, extraction and purification of therapeutic proteins, antibodies, and vaccines.
  • Agriculture and crop improvement.
  • Food additives research.
  • Functional nutraceuticals research.
  • Biodegradable materials development.

If you require large-scale preparation of plant proteins, CD BioSciences is an excellent choice. We are a leading biological company dedicated to the large-scale expression and purification of plant proteins. With our cutting-edge facilities and advanced technology, we offer a comprehensive solution for clients who need high-quality, mass-produced plant proteins. If you are interested in our services, please contact us for more details.

References

  1. Korf, U., et al. (2005) Large-scale protein expression for proteome research. Proteomics. 5(14):3571–3580.
  2. Sarramegna, V., et al. (2003). Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification. Cellular and molecular life sciences: CMLS. 60(8):1529–1546.

For research use only, not for clinical use.