Plant Protein Extraction Kit (Chlorophyll removal)

This kit is suitable for extracting total protein from various plant cell and tissue samples. The extraction process is simple and convenient. This kit contains a unique formulation that effectively solubilises cell membrane components, including cytoplasmic, nuclear and various organelle membranes. The kit contains a mixture of protease inhibitors that prevents protein degradation by proteases and ensures the extraction of high purity proteins.
The proteins extracted in this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation chromatography. The proteins extracted in this kit can be used in downstream protein research experiments such as WB, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity assay, Gel shift gel block assay, enzyme activity assay, etc.
The protein samples extracted from this kit contain high concentration of salt, so they cannot be used directly in 2D electrophoresis, and need to be desalted and then used in 2D electrophoresis. If the downstream experiments need to be used directly for isoelectric focusing and bidirectional electrophoresis, please use our kits with other product numbers.

Easy to use, protein extraction from cells, tissues does not need to go through pre-treatment such as grinding, repeated freezing and thawing, ultrasonic crushing.
The extraction process is simple and convenient, reducing the time of protein extraction to 1 hour.
Containing protein stabiliser, the extracted protein is stable.
Low background interference during UV detection of protein concentration.
Total protein extract contains a variety of active ingredients, which can fully release cytoplasmic, nuclear and membrane proteins, and can bind released proteins to prevent precipitation.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The Protease Inhibitor Blend contains six separate protease inhibitors AEBSF, Aprotinin, Leupeptin, Pepstatin A, Bestatin, E-64, each of which specifically inhibits one or more protease activities. The optimised composition of the mixture allows it to inhibit almost all essential protease activities.

Total Plant Protein Extract A 25mL/50mL
Protein Stabiliser B 200μL/400μL
Chlorophyll remover C 1mL/1mL×2
Protease inhibitor mixture D 100μL/200μL