Plant Protein Antioxidant Function Analysis Service

We are committed to becoming your reliable assistant and partner in the field of plant protein

  1. Home
  2. Services
  3. Plant Protein Function Analysis Services
  4. Plant Protein Medicinal Function Analysis Services
  5. Plant Protein Antioxidant Function Analysis Service
Services
Online Inquiry

Plant Protein Antioxidant Function Analysis Service

Inquiry

The imbalance between the endogenous antioxidant system and free radicals in the body's antioxidant process leads to oxidative stress and cellular damage, which results in many chronic diseases. Studies have shown that antioxidant peptides from plant protein sources have excellent antioxidant properties and safety, which can maintain the balance of free radicals, improve the body's ability to resist disease and slow down aging.

Figure 1. Oxidative stress component. – CD BioSciences Figure 1. Oxidative stress component. (Zhao, F., et al., 2022)

CD BioSciences provides services such as preparation, identification, activity assay, and action mechanisms analysis of plant-derived antioxidant peptides to help customers gain an in-depth understanding of plant antioxidant peptides' application value.

Service Content

Preparation service of plant antioxidant peptides based on enzymatic hydrolysis

We offer enzymatic hydrolysis to release antioxidant peptides from proteins of plants or plant derivatives. The enzymes we use include

  • Animal proteases: pepsin, trypsin
  • Plant proteases: papain, bromelain
  • Microbial proteases: alcalase, neutral proteases, and flavourzyme

We also provide microwave and ultrasound-assisted techniques to increase protein sensitivity to proteases and thereby increase peptide yields.

Plant antioxidant peptide purification service

To more accurately assess the structure and activity of antioxidant peptides, we offer purification methods including

  • Gel permeation chromatography (GPC)
  • Ion exchange chromatography (IEC)
  • Reverse phase high-performance liquid chromatography (RP-HPLC)
  • Membrane separation

Plant antioxidant peptide identification services

The identification of plant antioxidant peptides is usually done using MS and bioinformatics techniques. We offer the following identification methods

  • Fast atomic bombardment (FAB)
  • Electrospray ionization (ESI)
  • Matrix-assisted laser resolved ionization time-of-flight mass spectrometry (MALDI-TOF-MS)

Activity evaluation and mechanism analysis services of antioxidant peptides

Our service Details
In vitro chemical assays Free radical scavenging analysis. DPPH radical scavenging activity assay.
Hydroxyl radical scavenging activity assay.
Superoxide anion radical scavenging activity assay.
Metal ion chelation analysis.
Lipid peroxidation inhibition analysis.
In vivo animal research Analysis of regulation of endogenous antioxidant enzyme activity. Superoxide dismutase (SOD) assay.
Catalase (CAT) assay.
Glutathione peroxidase (GPx) assay.
Reduced glutathione (GSH) assay.
Malondialdehyde (MDA) assay.
Analysis of the regulation of antioxidant-related signaling pathways.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details. 

References

  1. Zhu, Y., et al. (2022). Food Protein-Derived Antioxidant Peptides: Molecular Mechanism, Stability and Bioavailability. Biomolecules.12(11):1622.
  2. Zhao, F., et al. (2022). Advances on the Antioxidant Peptides from Nuts: A Narrow Review. Antioxidants (Basel, Switzerland).11(10):2020.
  3. Fai-Chu, et al. (2020). Advances on the antioxidant peptides from edible plant sources. Trends in Food Science and Technology.99:44-57.

For research use only, not for clinical use.