Plant Protein Anti-Tumor Function Analysis Service
Cancer is the main threat to human health. During cancer development, genes undergo changes that evade cell apoptosis and affect the cell cycle. Plant proteins with potential anti-tumor properties have received widespread attention due to their promising therapeutic applications. Several plant proteins have been reported to have the ability to inhibit tumor growth, induce cancer cell apoptosis, and regulate cancer-related signaling pathways.
Figure 1. Plant immune protein RDR1 bioengineering in cancer therapy. (Qi, Y., et al., 2022) CD BioSciences is dedicated to the functional characterization of plant proteins. We offer various assays to evaluate the anti-tumor activity of plant proteins, providing valuable insights to help our clients understand the efficacy of plant proteins against cancer cells and their action mechanisms.
Service Content
Ectopic expression service of plant anti-tumor proteins
We can clone anti-tumor genes into lentiviral vectors to obtain stable induced cell lines for functional studies of plant anti-tumor proteins in animal cells. We offer Western Blot to verify protein expression in cell lines. In addition, we also provide lentiviral vectors expressing mCherry-fused proteins to observe the localization of the proteins.
Anti-tumor activity assay and action mechanism analysis service
Cell viability assay
We offer services to determine the cytotoxicity of plant proteins against tumor cells based on
- Colorimetric MTT assay
- Resazurin assay
- Trypan blue exclusion assay
- ATP-based assay
Apoptosis assay
Tumor cells have abnormal apoptotic processes and pathways, and our services help clients analyze the role of plant proteins in inducing apoptosis in tumor cells, including
- Electron microscopy examination
- Nuclear morphology staining
- Mitochondrial membrane potential assay
- Cell membrane surface phosphatidylserine assay
- Apoptosis molecular marker assay
- DNA damage detection
Cell cycle analysis
We offer flow cytometry-based cell cycle analysis services that can assess whether plant proteins induce tumor cell cycle arrest at specific stages.
Migration and invasion assay
We offer Transwell-based tumor cell migration and invasiveness assay measurements to help clients determine the inhibitory effects of proteins on tumor cell metastasis.
Angiogenesis assay
Angiogenesis is necessary for the growth of solid tumors. We provide services to evaluate the ability of plant proteins to inhibit tumor cell neovascularization, including
- Tube formation measurement
- Endothelial cell migration assay
MDR inhibition assay
MDR is a broad-spectrum drug resistance phenomenon in tumors leading to tumor recurrence. We provide services to evaluate the MDR reversal properties of plant proteins in tumor cells, such as
- P-glycoprotein (P-gp) expression analysis
- p53 expression analysis
- Bcl-2 family member expression level detection
| Plant protein types with anti-tumor functions have been discovered | |
| Lectin | Antibacterial peptide |
| Ribosome inactivation protein (RIP) | Plant immune proteins |
| Protease inhibitor | Cytotoxic enzymes |
CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.
References
- Qi, Y., et al. (2022). A plant immune protein enables broad antitumor response by rescuing microRNA deficiency. Cell.185(11):1888–1904.
- Guo, X., et al. (2010). Anti-tumor activity of a novel protein obtained from tartary buckwheat. International journal of molecular sciences.11(12):5201–5211.
- Ferriz-Martinez, et al. (2010). The role of plant lectins in cancer treatment. The New Approaches in the Treatment of Cancer. 71-89.
For research use only, not for clinical use.