Plant Plasma Membrane Protein Isolation Kit

Plasma membrane proteins make up only a small part of the total protein of plant cells, but play a crucial role in plant physiology. The isolation and purification of PM proteins from plant tissues has traditionally been done by sucrose density ultracentrifugation and water two-phase partitioning.

The plant tissue is first sensitized with buffer A, homogenized, and then passed through a specialized filter cartridge so that the homogenate passes in a zigzag path. During this process, the cell membrane breaks down into a predetermined size range. The protoplasmic membrane is separated from a mixture of unruptured cells, nuclei, organelles, and organelles by subsequent differential and density centrifugation without the use of ultracentrifugation. Because the same amount of starting material, specified centrifugal force, and specified duration are used for each experiment, the results are more consistent with high concentrations of PM protein. The whole process can be completed in about an hour.

Buffer A 25 ml
Buffer B 10 ml
Protein Extraction Filter Cartridges 50 units
Collection Tubes with Caps 50 units
Plastic Rods 2 units
Tissue Dissociation Beads 10 grams