Plant Pests Resistance Protein Function Analysis Service

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Plant Pests Resistance Protein Function Analysis Service

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Pests cause huge losses to agriculture. Using pesticides to control the spread of these pests is costly and harmful to the environment. Therefore, new strategies should be developed. The import of effective biomolecules into crops through genetic engineering techniques has made it easier to protect plants from pests with less contamination. 

Figure 1. Modeling the multilayered defense response of plants to pests. – CD BioSciences Figure 1. Modeling the multilayered defense response of plants to pests. (Hogenhout SA, Bos JI. 2011)

CD BioSciences is dedicated to the biology of plant adversity. We develop new plant pest resistance strategies for our clients and provide research services on the function of plant pest resistance proteins, including protein expression analysis, in vitro insect resistance activity testing, protein-insect interaction analysis, and identification of pest resistance mechanisms. 

Service Content

Plant Pest Resistance Gene Marker Service

Clarifying the genetic map of plant pest resistance genes can help improve the efficiency of plant breeding and understand the molecular mechanisms of disease resistance. Therefore, we offer the establishment of genetic maps with localization of major genes and QTLs. We can perform pathology testing and polymorphic genetic markers, both morphological and molecular, in segregating populations.

Plant Pest Resistance Gene Expression Analysis Service

We offer services to analyze gene expression patterns of pest-resistant proteins, including

  • Reverse transcription polymerase chain reaction (RT-PCR),
  • Quantitative real-time PCR (qPCR)
  • RNA sequencing (RNA-seq)

Gene expression analysis helps to identify plant genes that are up-regulated or induced in response to pest infestation, providing insight into their potential role in defense.

Analysis Service of In Vitro Pest Resistance Function

  • Analysis of contact poisoning effect.
  • Analysis of stomach action.
  • Analysis of repellent action.
  • Analysis of developmental and reproductive interference effects.

Plant-Pest Interaction Analysis Service

We offer yeast two-hybrid, CO-IP, and BiFC techniques to research the mechanisms by which plant proteins exert anti-pest activity, including

  • Analyzing interactions between plant proteins and pests physiology.
  • Analyzing interactions between plant proteins and pests biochemistry.
  • Analysis of interactions between plant proteins and pests behavior.

Transgenic Pest-Resistant Plants Research Service

We provide our customers with transgenic plants expressing pest-resistant proteins and evaluate the resistance.

Our methods
Agrobacterium-mediated Genetic gunnery
Polyethylene glycol Electroshock and laser
Microinjection Pollen tube passageway

Transgenic Pest-Resistant Plants Ecological Risk Analysis Service

We can analyze the potential impacts of transgenic pest-resistant plants on non-target organisms, ecology, and human health, and conduct risk assessments to ensure safe use of these pest-resistant proteins.

The types of proteins we can analyze Detail
Protease inhibitor, PI It is mainly found in storage organs such as plant tubers and seeds, where it interferes with larval growth and has inactivating activity against sensitive insects. Such as inhibitors of amino proteinases, cysteine proteases, metalloproteinases, or aspartyl proteases
α-Amylase inhibitors It is widely present in legumes or cereals and can block the digestive enzymes of pests.
Lectins It inhibits growth and development by binding to glycoproteins in the pest's digestive tract, hindering nutrient absorption.
Others Ribosome inactivating protein, chitinase, tryptophan dehydroxylase, lycopene, peroxidase, pea fat oxidase, polyphenol oxidase, and lipoxygenase.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details. 

References

  1. Hogenhout SA, Bos JI. (2011). Effector proteins that modulate plant-insect interactions.Curr Opin Plant Biol. 14(4):422-428.
  2. Arya, Surjeet & Shiva, Shatrughan & Upadhyay, Santosh. (2021). Entomotoxic Proteins from Plant Biodiversity to Control the Crop Insect Pests.

For research use only, not for clinical use.