Plant Nucleoprotein / Organelle Protein Extraction Kit

This kit is suitable for extracting nuclear and other organelle proteins from various plant cells and various solid plant tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient and can be completed within 1 hour. The prepared nuclear proteins are not only highly pure and maintain natural activity, but also have absolutely no cross-contamination.
The kit contains a unique formulation that effectively solubilises plant nuclear fractions. The protease inhibitor mixture contained in this kit prevents the degradation of proteins by proteases, which ensures the extraction of high purity proteins.
The proteins extracted in this kit can be used in downstream protein research such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel block assay, enzyme activity assay and so on.
The proteins extracted in this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelate chromatography. The protein samples extracted in this kit contain a high concentration of salt and cannot be used directly in 2D electrophoresis.
This kit uses non-enzymatic extraction, which makes the extraction process simple, convenient and fast, and can be completed within 1h, and there is absolutely no cross-contamination."

Reduce the time of protein extraction to 1 hour.
Contains protein stabiliser for stable extracted protein.
Low background interference in UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The Protease Inhibitor Blend contains six individual protease inhibitors; each inhibitor specifically inhibits one or more protease activities. The optimised composition of the mixture allows it to inhibit almost all essential protease activities.

Protein extract A 100mL/200mL
Plant nuclear protein extract B 15mL/30mL
Organelle Protein Extract C 15mL/30mL
Protease inhibitor mixture 250μL/500μL