Plant Nucleoprotein / Cytoplasmic Protein Extraction Kit (Non-enzymatic method)

This kit is suitable for the extraction of nuclear and other cytoplasmic proteins from a variety of plant cells and various solid plant tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient and can be completed within 1 hour. The prepared nuclear proteins are not only highly pure and maintain natural activity, but also have absolutely no cross-contamination.
The kit contains a unique formulation that effectively solubilises plant nuclear components. The protease inhibitor mixture contained in this kit prevents the degradation of proteins by proteases, which ensures the extraction of high purity proteins.
The proteins extracted in this kit can be used in downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking assay, and enzyme activity determination.
The proteins extracted in this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelate and chromatography. The protein samples extracted in this kit contain a high concentration of salt and cannot be used directly in 2D electrophoresis.
This kit adopts non-enzymatic extraction, the extraction process is simple and convenient, and the speed can be completed within 1 hour.

Reduce the time of protein extraction to 1 hour.
Contains protein stabiliser for stable extracted protein.
Low background interference in UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The protease inhibitor mixture contains six separate protease inhibitors AEBSF, Aprotinin, Leupeptin, Pepstatin A, Bestatin, E-64, each of which specifically inhibits one or more protease activities. The optimised composition of the mixture allows it to inhibit almost all essential protease activities, including serine proteases, cysteine proteases, aspartate proteases, alanyl-aminopeptidases and others.

Plant nucleoprotein extract A 50mL/100mL
Plant nucleoprotein extract B 10mL/20mL
Plant cytoplasmic protein extract C 2.5mL/5mL
Protease inhibitor mixture 250μL/500μL