Plant Nuclear Protein Extraction Kit (Non-enzymatic method)

This kit is suitable for extracting nuclear proteins from various plant cells and solid tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient and can be completed within 1 hour. The prepared nucleoproteins are not only of high purity and maintain natural activity, but also have absolutely no cross-contamination.
The kit contains a unique formula that can effectively solubilise plant nuclear components. The protease inhibitor mixture contained in this kit prevents the degradation of proteins by proteases, which ensures the extraction of high purity proteins.
The proteins extracted in this kit are active proteins with natural protein conformation, which can be used in downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking assay, and enzyme activity assay.
This kit does not contain EDTA and is compatible with downstream applications such as metal chelation chromatography. The protein samples extracted from this kit contain high salt concentration and cannot be used directly for 2D electrophoresis.

Contains protein stabiliser, the extracted protein is stable.
Low background interference in UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The Protease Inhibitor Blend contains six separate protease inhibitors AEBSF, Aprotinin, Leupeptin, Pepstatin A, Bestatin, E-64, each of which specifically inhibits one or more protease activities. The optimised composition of the mixture allows it to inhibit almost all essential protease activities.

Plant nucleoprotein extract A 100mL/200mL
Plant nuclear protein extract B 15mL/30mL
Protease inhibitor mixture 100μL/200μL