Plant Nuclear Protein Extraction Kit (No descaling agent)
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Plant Nuclear Protein Extraction Kit (No descaling agent)

Cat: PE00032
Size: 50T|100T
Shelf Life: 1 year
Datasheet:

Application

This kit is suitable for extracting nuclear proteins from various plant cells and various solid plant tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient. The prepared nucleoproteins are not only of high purity and maintain natural activity, but also have absolutely no cross-contamination.
This kit contains a unique formula that can effectively solubilise plant nuclear components. The protease inhibitor mixture contained in this kit prevents the degradation of protein by protease, which ensures the extraction of high purity protein.
The protein samples extracted from this kit contain high concentrations of salt and cannot be used directly in 2D electrophoresis.

Description

Contains protein stabiliser, the extracted protein is stable.
Low background interference in UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The Protease Inhibitor Blend contains six separate protease inhibitors AEBSF, Aprotinin, Leupeptin, Pepstatin A, Bestatin, E-64, each of which specifically inhibits one or more protease activities. The optimised composition of the mixture allows it to inhibit almost all essential protease activities, including serine proteases, cysteine proteases, aspartate proteases, alanyl-aminopeptidases etc.

Kit Composition

Plant nucleoprotein extract A 25mL/50mL
Phycobiliprotein Extract B 50mL/100mL
Plant nuclear protein extract C 15mL/30mL
Protease inhibitor mixture 100μL/200μL

Protein extracts 2 to 8°C, protease inhibitors -20°C, extract A is not used for a long time placed at -20°C.

For research use only, not for clinical use.