This kit can be used to extract mitochondrial membrane proteins from various plant samples in a simple and rapid way, the extraction process is simple and convenient, and high quality mitochondrial membrane proteins can be extracted within one hour. The kit contains a mixture of protease inhibitors and phosphatase inhibitors, which prevents the degradation of proteins by proteases and ensures the extraction of high-quality proteins. The membrane proteins extracted by this kit are naturally active, and the extracted proteins can be used in a variety of downstream protein research experiments such as Western Blotting, immunoprecipitation, and enzyme activity assays.
Reduce the time of protein extraction to 1 hour.
Contains protein stabiliser for stable extracted protein.
Low background interference in UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The Protease Inhibitor Blend contains six individual protease inhibitors; each inhibitor specifically inhibits one or more protease activities. The optimised composition of the mixture allows it to inhibit almost all essential protease activities.
Extract A 200mL/400mL
Extract solution B 25mL/50mL
Extraction solution C 250μL/500μL
Membrane Protein Lysis Solution 10mL/20mL
Protease inhibitor mixture 100μL/200μL
Protease inhibitors are stored at -20°C; extracts are stored at 2 to 8°C; membrane protein lysates are stored at room temperature.
For research use only, not for clinical use.