This kit is a high yield membrane protein extraction kit based on chemical rather than detergent methods. The Plant Membrane Protein Extraction Kit can extract membrane proteins from various plants, and can be used for crude preparation of purified membrane proteins and membrane protein preparation. The extraction process is simple and convenient. The kit contains a protease inhibitor mixture and a phosphatase inhibitor mixture, which prevents protein degradation by proteases and provides a guarantee of high quality protein extraction. The extracted proteins are used for bidirectional electrophoresis.
Contains protein stabiliser, the extracted protein is stable.
Low background interference in UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The Protease Inhibitor Blend contains seven individual protease inhibitors; each inhibitor specifically inhibits one or more protease activities. The mixture's optimised composition allows it to inhibit almost all essential protease activities, including serine proteases, cysteine acid proteases, aspartate proteases, alanyl-aminopeptidases, etc.
Membrane extraction solution A 25mL/50mL
Extraction solution B 250μL/500μL
Reagent C 10mL/20mL
Protease inhibitor mixture 100μL/200μL
Protein extracts and lysates are stored at 2~8℃; protease inhibitors are stored at -20℃.
For research use only, not for clinical use.