Membrane proteins can be combined or integrated into the cell membrane or organelles of the floorboard of the protein. Plant membrane protein accounts for about half of the film. The more complex the cell membrane, the more proteins it contains. Membrane proteins play important roles in plant growth, cell recognition, ion channel regulation, material transport function regulation, and plant environmental adaptation regulation. Membrane proteins can determine important functions and are required for the survival of organisms. Membrane proteins can be classified into three classes based on their location in the membrane, peripheral membrane proteins, integrated membrane proteins, and lipid-anchored proteins.

CD BioSciences is dedicated to the study of plant proteins. We offer our customers service with high-quality plant membrane protein preparation. It helps customers to further research plant membrane proteins.

Preparation Method of Plant Membrane Protein

• Extraction of plant membrane protein by conventional gradient centrifugation (SGU)
• Extraction of plant membrane protein by chromatography of membrane protein, CMP
• Extraction of plant membrane protein by sequential extraction method
• Separation of plant membrane protein by two aqueous phase affinity separation
• Separation of plant membrane protein by centrifugal column method
• Separation of plant membrane protein by biotin-avidin affinity method
• Selective separation of plant membrane protein with special detergents

Figure 1. Phase separation of detergents is used to purify membrane proteins. (Arnold T, et al. 2007)

Application Fields

• Assay of enzyme activity on membrane
• New drug discovery
• Membrane protein antibody production
• Research of interaction between proteins
• Research the mechanism of G protein-coupled receptor involved in cell signal transduction

Other Services

Due to the low protein content in the membrane and the dilution of the protein during preparation, it is necessary to concentrate the membrane protein for preservation and research. Our concentration methods,

• Evaporation under reduced pressure and heating
• Evaporation from the airflow
• Freezing method
• Absorption method
• Ultrafiltration method

Screening of detergents for dissolving integrated membrane proteins. Integrated membrane proteins are extremely hydrophobic and usually require high concentrations of detergents capable of solubilizing but not inactivating membrane proteins.

Our Advantages

• We can extract membrane proteins from tissues with low protein content.
• Our membrane protein extraction services minimize damage to membrane structure.
• We have a variety of solubilizing surfactants available to increase protein concentration during membrane protein extraction.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.

For research use only, not for clinical use.