Plant Heavy Metal Resistance Protein Function Analysis Service
Heavy metals (HMs) have toxic effects on plants, which can damage plant lipids, cell membranes, and DNA, oxidize proteins, and inactivate enzymes. Therefore, plants evolve corresponding response proteins during long-term evolution, participating in the resistance to HM stress.
CD BioSciences's services include the identification of HM-resistant proteins, the determination of HM resistance in transgenic plants, and the analysis of action mechanisms. Our services help clients to better understand the function of plant proteins in HM detoxification and provide solutions for bioremediation of HM contamination.
Figure 1. The impact of HMs toxicity on plants(A), plant response pathways(B), and omics methods(C). (Khan, M.I., et al., 2021) Service Content
Identification HMs Stress Response Protein Service
We provide plant protein extraction service based on trichloroacetic acid/acetone precipitation or phenol. We assist clients in analyzing plant proteomic changes induced by HM, utilizing techniques including
- Two-dimensional gel electrophoresis (2-DE)
- Mass spectrometry (MS) protein identification
Transgenic Plants HMs Resistance Function Analysis Service
We can process, knock out, and shield plant genes through transgenic technology. The function of the gene is then analyzed by corresponding HM stress experiments.
- Assay the accumulation of methylglyoxal (MG) and reactive oxygen species (ROS).
- Assay main root length and seed germination rate.
- Assay nitrogen assimilation efficiency.
- Assay carotenoid and chlorophyll content.
- Assay photosynthetic rate and stomatal conductivity.
- Assay defense protein (CAT, POD, SOD) activity.
- Analysis of absorption and transport capacity of mineral elements such as Ca and Mg.
Plant Protein HMs Resistance Mechanism Analysis Service
The detoxification function of plants towards HMs lies in the existence of a precise and complex regulatory mechanism. We provide customers with action mechanism analysis services through preliminary analysis binding protein interaction experiments. Our services include detecting whether proteins are involved in
- Promoting ectomycorrhizal growth and limiting metal movement.
- Promoting root secretion synthesis.
- Protecting plasma membrane integrity.
- Promoting the synthesis of membrane transport proteins.
- Promoting the synthesis of thiol-containing chelating compounds for vesicle isolation.
- Induces molecular chaperones to reconstitute proteins.
- Scavenges ROS.
- Regulates photosynthesis and mitochondrial respiration.
| Protein types we can analyze | Details |
| HM transporter proteins | ATP-binding cassette, HMA family, CAX cassette, CDF family, Nramp family, ZIP family, CTR family, MATE family, YSL family |
| Osmoregulatory proteins | Proline, soluble proteins |
| HM chelating proteins | Metallothioneins (MTs), plant chelating peptides |
| Heat shock proteins | HSPs 70, HSPs 60, HSPs 90, HSPs 100, sHSPs |
CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.
References
- Khan, M.I., et al. (2021). Regulatory hubs and strategies for improving heavy metal tolerance in plants: Chemical messengers, omics and genetic engineering. Plant physiology and biochemistry: PPB, 164:260-278.
- Hossain Z, et al. (2013). Contribution of proteomic studies towards understanding plant heavy metal stress response. Front Plant Sci. 3:310.
- Ghori, NH., et al. (2019). Heavy metal stress and responses in plants. Int. J. Environ. Sci. Technol. 16:1807-1828.
For research use only, not for clinical use.