Plant Genetic Variation Assay Service
CD BioSciences provides comprehensive plant genetic variation assay services based on whole-genome resequencing technology of plants. Sequencing and differential analysis of the genome of an individual or a group of species through high-throughput sequencing technology can obtain a large amount of genetic variation information, which can be used to develop molecular markers and establish a genetic polymorphism database. Our service lays the foundation for the subsequent revelation of evolutionary relationships, the mining of functional genes, and the development of high-quality crop varieties.
Figure 1. Schematic diagram of a high-throughput sequencing method for genetic diversity assessment. (Loera-Sánchez M, et al. 2019)Service Content
Service process
Molecular marker services
Our molecular marker technology is widely used for genetic fingerprinting, hybrid identification, and functional genomics. In crop breeding, we help clients identify and select desired genotypes at an early stage. In addition to sequence-based SNP markers, we also provide many types of molecular markers, including
| RFLP | RAMP | CAPS | iPBS |
| RAPD | SRAP | IRAP | RAD |
| AFLP | ISSR | REMAP | SFP |
| SSR | TRAP | RBIP |
Other genetic diversity assessment marker services
In addition to molecular markers, we offer the following methods to help clients assess the degree of similarity and difference between crop germplasm.
- Morphological markers
We provide morphological marker services to develop multiple genotypes with stress tolerance attributes for breeders based on the growth stage dependence of the crop.
- Nutritional growth
Germination rate, number of leaves, number of nodes, leaf area index, leaf colour, stem thickness, etc.
- Reproductive growth
Flowering days, maturity days, flower colour, morphology, sugar value,etc.
- Maturity stage
plant height, yield, dry weight, and grain weight, etc.
- Cytological markers
We can physically map nuclear content directly on chromosomes and identify the protein content of cells. We offer GISH and FISH techniques to characterise foreign gene infiltration in crop species and to dissect the genetic composition of natural and artificial hybrids.
- Biochemical markers
Our biochemical labelling service, based on protein/isozyme analysis, is one of the simplest and fastest methods available and is ideal for projects requiring low-level genetic diversity estimation.
Sample Requirements
| Sample types | Requirements |
| Genomic DNA |
|
| Cells | ≥ 5×106 cell. |
| Plant tissue | ≥ 200mg. |
CD BioSciences specializes in plant genetic variation assay services. Our cutting-edge testing methods, including high-throughput sequencing and molecular marker technologies, allow us to identify and assess genetic variation on both an individual and genome-wide scale. If you have plant breeding research requirements, please contact us for more information.
References
- Loera-Sánchez M, et al. (2019). DNA-Based Assessment of Genetic Diversity in Grassland Plant Species: Challenges, Approaches, and Applications.Agronomy. 9(12), 881.
- Martínez-Fortún, J., et al. (2022). Natural and artificial sources of genetic variation used in crop breeding: A baseline comparator for genome editing. Frontiers in genome editing. 4, 937853.
- Sadia, B., et al. (2021). Exploring Plant Genetic Variations with Morphometric and Molecular Markers. In Genetic Variation.
- Molina, C., et al. (2023). ddRADseq-mediated detection of genetic variants in sugarcane. Plant molecular biology. 111(1-2), 205-219.
For research use only, not for clinical use.