Plant Genetic Transformation Service

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Plant Genetic Transformation Service

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CD BioSciences provides professional plant genetic transformation services, using recombinant DNA, cell tissue culture, and germplasm system transformation technologies to purposefully insert exogenous genes or DNA fragments into the recipient plant genome and obtain new plants.

Our one-stop service is the whole process of transgenic plants, including target gene fragments extraction, construct vectors, vector transformation, integration of target genes into the plant's nuclear genome, and transgenic plant validation.

Schematic diagram of T-DNA transfer and integration into the plant genome. Figure 1. Schematic diagram of T-DNA transfer and integration into the plant genome. (Su, W., et al. 2023)

Service Content

Service process

  • Plasmid modification and vector construction.
  • Plant cultivation and sterile seedling obtainment.
  • Plant genetic transformation.
  • Positive screening and plant regeneration.
  • PCR detection of exogenous genes.

Plant genetic transformation service

Our plant genetic transformation technology is divided into two categories. The direct gene transfer technology, including the gene gun method, protoplast method, liposome method, pollen tube channel method, electro-excitation transformation method, and PEG-mediated transformation method. The other is biologically mediated transformation methods, mainly Agrobacterium-mediated and virus-mediated transformation methods.

Common methods we offer Details
Agrobacterium-mediated method Short cycle time, high transformation rate, less gene silencing phenomenon, large transformed fragments, and obvious insertion fragments, suitable for dicotyledonous plants.
Gene gun method Short transformation time, almost no requirement of recipient plants, but not conducive to the stable expression and inheritance of exogenous DNA.
Pollen tube channel method No tissue culture process, but poor reproducibility.
Electroshock method No host restriction, but a long cycle time.
PEG-mediated transformation Widely used, but requires protoplasts and high environmental requirements.

Genetically Transformable Plant Types

Types Details
Model plants Tobacco, Arabidopsis thaliana.
Cash crops Cotton, soybeans, cucumbers, tomatoes, peppers, canola, kale, apples, grapes, watermelons, melons, citrus, alfalfa, cabbage, lettuce, etc.
Food crops Rice, wheat, maize, potato,etc.

Species not listed can be developed according to the requirements of our clients, who can make personalized requests for the projects. For specific genotypes of the transformable species and the number of T0 generation-positive plants available, please online inquire.

Notification for Sample Preparation and Handling

  • Clients can directly provide the constructed transformation vector, or entrust our company to carry out the relevant vector construction service.
    • Requirements of plasmids provided by clients.
      1) Concentration 80-100ng/μL (plasmid without degradation).
      2) Volume ≥ 10μL.
      3) Ice bag or dry ice transport.
    • Requirements of E. coli/Agrobacterium provided by clients.
      1) Bacterial solution within 1 year, preserved with glycerol.
      2) Bacterial plate activated within one week.
  • Matured seeds ≥ 100 seeds with ≥ 90% germination within one year.

CD BioSciences has a professional and technical team that has been engaged in plant genetic transformation for many years. Our plant genetic transformation platform can help scientists research gene function and breed excellent plant varieties. If you are interested in our services, please contact us and we will provide comprehensive service support.

References

  1. Su, W., et al. (2023). Technological Development and Application of Plant Genetic Transformation. International journal of molecular sciences. 24(13), 10646.
  2. Keshavareddy, G et al. (2018). Methods of Plant Transformation- A Review. International Journal of Current Microbiology and Applied Sciences. 7. 2656-2668.
  3. Anjanappa, R. B., & Gruissem, W. (2021). Current progress and challenges in crop genetic transformation. Journal of plant physiology. 261, 153411.

For research use only, not for clinical use.