Plant Gene Overexpression Service

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Plant Gene Overexpression Service

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CD BioSciences provides gene overexpression technology as an alternative or complementary method for plant gene loss of function, including plant gene overexpression vector construction and genetic transformation services. We can obtain higher amounts of target mRNA transcript levels and protein expression levels in plant cells, and investigate the function of genes through phenotypic analyses.

Figure 1. Overexpression of biotic stress-inducible genes activates heat and drought resistance in tobacco. – CD BioSciences  Figure 1. Overexpression of biotic stress-inducible genes activates heat and drought resistance in tobacco. (Stavridou, E., et al. 2021)

Service Content

Service process

Service process

Plant gene overexpression vector construction service

With the improvement of science and technology, higher requirements have been put forward for vectors. The vectors we provide have high copy numbers, strong promoters, high isolation and structural stability, and a wide range of hosts, etc.

  • Promoter selection service
Type Promoters we provide
Constitutive promoters such as p35s, pUbi, pActin1 and pNos.
Tissue-specific promoters such as pGSSP, pRBCS (leaf), pAP3 (flower), C4Pdk (green tissue), pPG1090 (fruit), napinB (seed), mas2, and pPYK10 (root).
Inducible promoters such as pCAB (light), pRD29A (stress), and pER (estradiol).
  • Artificial construction of vector plasmids

To transport DNA molecules to recipient plant cells, we provide customers with artificially constructed plasmids as carriers, which possess the following elements

  1. The replication start site Ori refers to the site that controls the replication start.
  2. Antibiotic resistance genes, such as Amp and Kan.
  3. Multiple cloning sites carrying exogenous gene fragments.
  4. P/E promoter/enhancer.
  5. Terms termination signal.
  6. Add poly (A) signal to stabilize mRNA.

Plant genetic transformation

After the vector construction is completed, we provide a platform for plant genetic transformation, including monocotyledonous and dicotyledonous plants, such as tobacco, Arabidopsis, wheat, rice, barley, soybeans, potatoes, cucumbers, and most of the transformation systems are within our scope.

Plant phenotype and gene function analysis

We overexpressed plant genes to increase the abundance of the expression products. Then, we can analyze the function of the gene by comparing the phenotypic differences between the overexpressed material and the wild-type plants.

Sample Requirements

Sample type Requirements Details
Plasmids >20μg Sequencing results and vector resistance.
E. coli (glycerol bacteria) >500μL Sequencing results, bacterial fluid resistance, and within three months.
Seeds >25 grain Germination rate > 90%.
Seed harvested within one year.
Plant tissues >3g Fresh and tender green. 
Rapid liquid nitrogen treatment after sampling.
cDNA >20μL Non-polluting and can amplify housekeeping genes.
gDNA >20μg OD260/280=1.8-1.9, OD260/230=1.9-2.2.

Deliverables

  • Overexpression vector plasmid (containing full sequence information and annotation information), one copy of E. coli bacteriophage, and one copy of Agrobacterium bacteriophage (25% glycerol bacteriophage).
  • Sequencing results of the assay.
  • Experimental report (experimental procedure, results of bioinformatics interpretation of overexpression plants).
  • Corresponding number of overexpression plants and control plants.

CD BioSciences is a pioneering biological company specializing in plant gene overexpression services. With our proficiency and expertise in gene manipulation, we provide customized solutions to amplify the expression of target genes in plants. If you are interested in our services, please contact us for reliable and customized gene overexpression services.

References

  1. Stavridou, E., et al. (2021). Overexpression of A Biotic Stress-Inducible Pvgstu Gene Activates Early Protective Responses in Tobacco under Combined Heat and Drought. International journal of molecular sciences. 22(5), 2352.
  2. Capriotti, L., et al. (2020). Biotechnological Approaches: Gene Overexpression, Gene Silencing, and Genome Editing to Control Fungal and Oomycete Diseases in Grapevine. International journal of molecular sciences. 21(16), 5701.
  3. Nguyen, Q. H., et al. (2019). Overexpression of the GmDREB6 gene enhances proline accumulation and salt tolerance in genetically modified soybean plants. Scientific Reports. 9(1).

For research use only, not for clinical use.