Plant Cytoplasmic Protein Extraction Kit

This kit is suitable for the extraction of cytoplasmic proteins from various plant cells and various solid plant tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient and can be completed within 1 hour. The prepared proteins are not only of high purity, but also maintain natural activity.
The proteins extracted in this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelate chromatography. The proteins extracted in this kit can be used in protein research such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking assay, and enzyme activity assay.
This kit adopts non-enzymatic extraction, the extraction process is simple and convenient, fast, can be completed within 1 hour, and there is absolutely no cross-contamination.
The protein extracted in this kit contains high concentration of salt and cannot be used directly in 2D electrophoresis.

Reduces the time of protein extraction to 30 minutes to 1 hour.
Contains a protein stabiliser and the extracted protein is stable.
Low background interference in UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The Protease Inhibitor Blend contains six individual protease inhibitors; each inhibitor specifically inhibits one or more protease activities. The optimised composition of the mixture allows it to inhibit almost all essential protease activities.

Protein extract A 50mL/100mL
Plant cytoplasmic protein extract B 5mL/10mL
Protease inhibitor mixture 250μL/500μL