Plant Chloroplast Membrane Protein Extraction Kit
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Plant Chloroplast Membrane Protein Extraction Kit

Cat: PE00013
Size: 50T|100T
Shelf Life: 1 year
Datasheet:

Application

This kit is a simple and rapid method to extract chloroplast membrane proteins from various plant samples. The extraction process is simple and convenient, and high quality chloroplast membrane proteins can be extracted within one hour. The kit contains a unique formulation that can effectively solubilise membrane components. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases, which ensures the extraction of high purity proteins.
The proteins extracted in this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation chromatography. The proteins extracted in this kit can be used in downstream protein research experiments such as WB, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity assay, Gel shift gel block assay, enzyme activity assay, and so on.
The protein samples extracted in this kit contain high concentration of salt components and cannot be used directly in 2D electrophoresis.
This kit adopts non-enzymatic extraction, the extraction process is simple and convenient, and the speed can be completed within 1 hour.

Description

Easy to use, extracted protein does not need to go through pre-treatment such as grinding, repeated freezing and thawing, ultrasonic crushing and so on.
The extraction process is simple and convenient, reducing the time for protein extraction to 1 hour.
Contains protein stabiliser, the extracted protein is stable.
Low background interference during UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The Protease Inhibitor Blend contains six individual protease inhibitors; each inhibitor specifically inhibits one or more protease activities. The optimised composition of the mixture allows it to inhibit almost all essential protease activities.

Kit Composition

Chloroplast Extract A 100mL/200mL
Chloroplast Extract B 25mL/50mL
Membrane Protein Lysis Solution C 10mL/20mL
Protease inhibitor mixture 100μL/200μL

Protein extracts and lysates are stored at 2~8℃; protease inhibitors are stored at -20℃.

For research use only, not for clinical use.