Plant Antifungal Protein Function Analysis Service

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Plant Antifungal Protein Function Analysis Service

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Fungi and mycotoxins cause crop yield reduction and jeopardize human health. Therefore, during the long-term struggle against pathogenic fungi, plants have formed many antifungal proteins or peptides, such as protease inhibitors (PI), ribosome-inactivating proteins (RIP), chitinases, glucanases, etc. Research on these proteins is beneficial in improving the disease resistance of crops.

CD BioSciences provides protein analysis services for plant antifungal disease resistance. Our services include protein expression purification, expression analysis, in vitro disease resistance testing, enzyme inhibition activity analysis, and transgenic plant disease resistance assay. We provide solutions based on the client's experimental objectives, laying the foundation for the utilization of plant resources.

The mode of action of plant antifungal proteins. Figure 1. The mode of action of plant antifungal proteins. (Chiu, T., et al. 2022)

Service Content

Plant Antifungal Protein Expression and Purification Service

We provide plant antifungal protein expression services for customers. Then, the fractions are purified by ion exchange chromatography and the purification effect is identified on SDS-PAGE.

Plant Antifungal Protein Concentration Assay and Amino Acid Sequencing Service

We provide matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to determine protein purity. We also provide amino acid sequencing services based on LC-MS/MS to further identify the expressed proteins.

In Vitro Antifungal Test Service

Our methods Description
Microbial dilution method Our service examined thein vitro inhibitory activity of the proteins against fungi by evaluating the growth of the fungi through the optical density (OD) values of the cultures. The method applies to fungi that can be cultured in liquid mediums and have distinct colors.
Mycelial growth rate method We provide protein inhibition assays for the growth of corresponding fungal mycelium. The assay is suitable for fungi that require mycelium to extend well on the surface of the solid medium.

Protease Inhibitory Activity Characterization Service

Pathogenic fungi secrete various enzymes to decompose plant cells during the process of infecting plants. Therefore, plants inhibit the activity of these enzymes by producing enzyme inhibitors. Our services include assay inhibitory effects of aspartic acid, serine, cysteine, and metalloproteinases, as well as the calculation of IC50 values.

Genetically Modified Plants Antifungal Activity Determination Service 

  • Production of transgenic plants.
  • Restore transgenic genes in T1 generation.
  • Assay antifungal activity in T1 plant nutrient tissues.
  • Assay antifungal activity in seeds.
  • Assay specificity of antifungal activity.

Screening and Validation Service for Plant Antifungal Protein Interaction Targets

We provide yeast two-hybrid and MS identification protein candidate interaction targets and validate the interaction through a series of in vitro andin vivo experiments to elucidate the action mechanism of plant antifungal proteins.

The types of proteins we can analyze Detail
Lectins, inhibitors of trypsin, chymotrypsin, and cysteine protease Inhibit the physiological metabolism of fungi.
Chitin binding protein, chitinase, glucanase Degrade fungal cell walls.
Thaumatin-like protein (TLP), phospholipid transporter protein Affect fungal cell membranes.
Ribosome inactivation protein (RIP) Destroy fungal ribosomes.
PR-10 protein, RNA hydrolase type ribosome deactivation protein Degrade fungal nucleic acids.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details. 

Reference

  1. Chiu, T., et al. (2022). The potential of plant proteins as antifungal agents for agricultural applications. Synthetic and systems biotechnology. 7(4):1075–1083.

For research use only, not for clinical use.