Plant Antifreeze Protein Recrystallization Inhibition Assay Service

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Plant Antifreeze Protein Recrystallization Inhibition Assay Service

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Ice recrystallization occurs when the sample remains below its melting point (Tm). This process realizes the redistribution of ice crystals. Recrystallization is harmful to the ultrastructure and hydration of plants. Therefore, most overwintering plants contain antifreeze proteins (AFPs). AFPs can inhibit ice recrystallization, which helps plants survive during freezing and thawing processes.

CD BioSciences has professional knowledge and experience in plant stress biology research. We provide recrystallization inhibition (RI) detection services for AFPs based on the splat cooling assay (SCA), sucrose sandwich assay (SSA), and capillary method. We help customers further analyze the performance of AFPs to expand their applications in medical and food storage, as well as crop variety improvement.

Ice recrystallization inhibition function of AFPs. Figure 1. Ice recrystallization inhibition function of AFPs. (Eskandari A, et al. 2020)

Service Content

Splat Cooling Assay (SCA) Service

We provide a splat cooling method to detect the RI of AFPs, which can be used to detect the endpoint of RI. However, this method can only observe one sample at a time, and the recorded images can only be compared after assembly. Furthermore, the sample cannot be stored.

Sucrose Sandwich Assay (SSA) Service

Our sucrose sandwich method is widely used to research plant AFPs and their RI, which can be used for semi-quantitative analysis of RI endpoints by continuously diluting the sample. It can be used as the basic method for screening AFPs in samples. However, this method requires a longer annealing time and is more time-consuming.

Capillary Assay Service

We assay the RI of AFPs through glass capillaries. This method can simultaneously observe 10-15 samples, visually compare a series of samples in the same field of view, and allow sample archiving. It is also suitable for rapid screening of AFPs in column chromatography fractions. 

Other Service

Ice crystal image analysis.

Evaluation of ice recrystallization rate.

Evaluation of ice volume fraction.

Notification for Sample Preparation and Handling

Sample Type Sample Recommended
Protein freeze-dried powder Total protein content > 100µg
Protein solution Total protein content > 150µl

Protein concentration > 1mg/ml.

Protein purity > 90%.

Samples should be as salt-free as possible.

Please add sufficient dry ice during transportation.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details. 

References

  1. Eskandari A, et al. (2020). Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture. Biomolecules. 9;10(12):1649.

  2. Deswal, R., & Sharma, B. (2014). Antifreeze Proteins in Plants: An overview with an insight into the detection techniques including nanobiotechnology. Journal of Proteins & Proteomics, 5.

For research use only, not for clinical use.