Plant Antibody Phage Display Platform

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Plant Antibody Phage Display Platform

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CD BioSciences's plant antibodies phage display platform includes single-stranded filamentous phage, λ-phage, T4-phage, and T7-phage display systems. Plant monoclonal antibodies (mAbs) screening through our platform is fast, easy, and efficient.

What is Phage Display?

The phage display platform utilizes genetic engineering technology to attach the antibody's gene to phages and display it as a fusion protein on the phage's surface. Then, using the target molecule in combination with appropriate elution methods, remove the non-specifically bound phage and obtain antibodies with high affinity to the target.

Phage display methodology. Figure 1. Phage display methodology. (Almagro, J. C., et al., 2019)

Platform Content

Single-stranded filamentous phage display system

  • pIII display system
pIII display system.

The pIII display system is commonly used for the preparation of phage antibodies. Compared with the hybridoma technique, the pIII display mimics the natural immune selection system, the screening range is extended to millions or even hundreds of millions, and the time is shortened to a few weeks.

  • pVIII display system
pVIII display system.

The pVIII is the major shell protein of Ff phage with a molecular weight of 5.2 kDa and about 2700 copies per virus particle. Due to the high copy number of the pVIII protein, this system is more suitable for screening low-affinity ligands.

λ phage display system

λ phage display system.

The genome of the λ phage is a 48.5 kb linear double-stranded DNA molecule. It is assembled inside the host cell without the requirement to secrete exogenous peptides or proteins outside the bacterial cell membrane, and it can display active large-molecule proteins (more than 100 kDa) and toxic proteins, with an extremely wide range of applications.

T4 phage display system

T4 phage display system.

The T4 phage capsid has two non-essential capsid proteins, SOC (small outer capsid protein) and HOC (highly antigenic outer capsid protein). The advantage of T4 phage is that it can realize the simultaneous display of both the SOC site and HOC site, and the number of copies to be displayed is also higher.

T7 phage display system

T7 phage display system.

The 10B protein is present on the T7 phage surface and is commonly used to construct phage display systems. It can display peptides of 50 amino acids in high copy, peptides or proteins of 1200 amino acid residues in low copy (0.1-1/phage), or medium copy (5-15/phage). Therefore, it is widely used to screen proteins with different molecular weights and affinities.

Advantages and Limitations

Advantages Limitations
  • High-throughput screening with high efficiency.
  • Easy to purify and analyze sequences.
  • Screening time is short and can avoid the artificial immunization stage.
  • Enables preparation of antibodies to antigens that are not immunogenic and highly toxic.
  • Limited peptide library capacity.
  • Dependent on intracellular gene expression.
  • Unnatural pairing of heavy and light chains.
  • Antibody form dominated by Fab or scFV.

CD BioSciences has been deeply involved in the field of antibody discovery for many years and has built a mature phage display technology platform. We provide a comprehensive one-stop service based on our rich expertise and cutting-edge instruments. If you are interested in our platform, please contact us. Our experienced scientists are glad to satisfy every client's requirements and provide appropriate solutions.

Reference

  1. Almagro, J. C., et al. (2019). Phage Display Libraries for Antibody Therapeutic Discovery and Development. Antibodies (Basel, Switzerland).8(3), 44.

For research use only, not for clinical use.