Plant Antibacterial Protein Function Analysis Service

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Plant Antibacterial Protein Function Analysis Service

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During the process of infection, the conserved substances of bacteria are recognized by immune receptors on the plant cell membrane, thereby stimulating the plant's immune response. However, many bacteria have evolved effector proteins that interfere with the host immune system and enhance its pathogenicity. Therefore, revealing the mechanism of pathogenic bacteria infecting plants is of great significance for preventing and controlling these microorganisms in production.

CD BioSciences focuses on the functional analysis of plant proteins, and we provide research services on the function of plant antibacterial proteins. Our services include gene expression detection, localization analysis, and antibacterial activity testing. We provide customers with a one-stop service to help them gain a deeper understanding of the protein action mechanism. 

Formation of active pattern recognition receptor complexes. Figure 1. Formation of active pattern recognition receptor complexes. (Dodds, P. N., & Rathjen, J. P. 2010)

Service Content

Plant Antibacterial Gene Expression Detection Service

The expression level of target genes in plants determines their functional strength. We provide customers with expression measurement services based on RT qPCR, Southern blot, and Western blot.

Localization and Full-length Identification Service of Plant Antibacterial Gene

We provide techniques such as FISH, GFP fusion protein, and nucleocytoplasmic separation to explore the specificity of protein expression between tissues or subcellular cells. We also provide 5'/3' RACE technology for studying the accuracy of sequences and whether there are new variable cuts.

In Vitro Antibacterial Test Service

Our methods Description
Plate counting The antibacterial effect is characterized by the survival or mortality rate of bacteria. Our service can measure dose-dependent bactericidal efficacy, suitable for both Gram-negative and Gram-positive bacteria.
LIVE/DEAD staining LIVE/DEAD staining is a commonly used method to characterize the antibacterial effect. Protein treats the bacteria then staining them. If the bacterial cell membrane is damaged, it changes color, indicating bacterial death.
Scanning electron microscopy characterization After treating bacteria using antibacterial proteins, some bacterial forms may change, such as wrinkles, dents, or damage. Therefore, we provide scanning electron microscopy to observe the morphology of bacteria and characterize the antibacterial effects.

Removal of Bacterial Biofilm Testing Service

Biofilm is the main reason why bacterial diseases are difficult to cure. Therefore, it is necessary to evaluate the inhibitory effect of antibacterial proteins on bacterial biofilms. Our services including

  • Analysis of the impact on bacterial biofilm formation.
  • Analysis of the impact on the permeability of bacterial biofilms.
  • Analysis of initial adhesion of inhibiting bacteria to attached surfaces.
  • Degradation analysis of bacterial biofilm matrix.

In Vivo Study of Antibacterial Function

We provide transgenic technology to overexpress the target gene in plants. We also use RNAi or CRISPR/Cas9 to silence or mutate the target gene. Then, the function of the target protein is verified through corresponding bacterial infection experiments.

Antibacterial targets. Figure 2. Antibacterial targets. (Khameneh B, et al. 2019)
The types of proteins we can analyze Detail
Transglycosylase, transpeptidase Inhibition of bacterial cell wall biosynthesis.
Plant antimicrobial peptides (PAMPs), lectin Destruction of bacterial biofilm.
Ribosome inactivation protein (RIP) Inhibition of bacterial protein biosynthesis, such as Abrin, Crotin II, Curcin II, Luffin, Modeccin, Ricin, Viscumin, etc.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details. 

References

  1. Dodds, P. N., & Rathjen, J. P. (2010). Plant immunity: towards an integrated view of plant-pathogen interactions. Nature reviews. Genetics. 11(8): 539–548.
  2. Khameneh B, et al. (2019). Review on plant antimicrobials: a mechanistic viewpoint. Antimicrob Resist Infect Control. 8:118.

For research use only, not for clinical use.