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pJKW0458
Cat: PP00762
Vector type: Plant Expression, CRISPR
Datasheet:
Product Info
Vector backbone
pEarleyGate100
Modifications to backbone
p35S:CAS9 in pEarleyGate 100
3xFLAG::NLS::humanized CAS9::NLS (from pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene 42230)) was PCR amplified using pX330 as template, and cloned into Xho1 and Xba1 digested pEARLEYGATE100 by Gibson assembly.
Hind III and SbfI sites can be used for cloning in the AtU6-26 sgRNA fragment.
3xFLAG::NLS::humanized CAS9::NLS (from pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene 42230)) was PCR amplified using pX330 as template, and cloned into Xho1 and Xba1 digested pEARLEYGATE100 by Gibson assembly.
Hind III and SbfI sites can be used for cloning in the AtU6-26 sgRNA fragment.
Bacterial Resistance(s)
Kanamycin, 50 μg/mL
Growth Temperature
37℃
Growth Strain(s)
NEB Stable
Copy number
Unknown
For research use only, not for clinical use.