PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5 | Aquaporins
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PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5 | Aquaporins

Cat: PA00740
Size: 100 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Nicotiana tabacum, Phoenix dactilifera, Raphanus sativus, Zea mays
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from N terminus of Raphanus sativus PAQ1O80368. Chosen peptide is conserved in PIP1;1, PIP1;2, PIP1;3 N-terminus of Raphanus sativus and in  all 5 isoforms of Arabidopsis thaliana coded by:AT3G61430.1 (PIP1;1), AT2G45960.3 (PIP1;2), AT1G01620.1 (PIP1;3), AT4G00430.1 (PIP1;4), AT4G23400.1 (PIP1;5)
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. For long time storage over 1 year please dilute the antibody solution 10 fold with 10 mM Tris-buffered saline containing 10% glycerol. Glycerol may suppress denaturation of the protein.
Application
ELISA (ELISA), Western blot (WB)
Recommended dilution
1 : 8000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW
30.66 | 28 kDa (Raphanus sativus)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Nicotiana tabacum, Phoenix dactilifera, Raphanus sativus, Zea mays
Predicted reactivity
Lupinus sp. , Hordeum vulgare, Solanu lycopersicum, Ricinus communis, Oryza sativa, Populus trichocarpa
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable. Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient. PIP1;1-PIP1;5 isoforms can be detected in most tissues.,Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Antibody reacts also with PIP2 isoforms in Zea mays.

Description

PIPs proteins are aquaporins that facilitate the transport of water and small neutral molecules across cell membranes. PIP1; 2 is also known as PIP1B.

For research use only, not for clinical use.