Antibodies will detect target protein in a few µg of a crude preparation loaded per well, If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient,,Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel,
PIPs proteins are aquaporins that promote the transport of water and small neutral molecules across cell membranes, optional names :PIP1-1, PIP1-2, PIP1-3, intrinsic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin 1, PIP1, PIP1 Transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B. DyLight 594 has an Amax = 593 nm and an Emax = 618 nm. DyLight is a registered trademark of Thermofisher Inc. and its subsidiaries.
For research use only, not for clinical use.