PIF5 | Phytochrome interacting factor 5 (rabbit antibody)

Inquiry

Cat: PA01358

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIF5, UniProt: Q84LH8, TAIR: AT3G59060

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

49.3 | 62 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Not reactive in

Physcomitrella patens, Solanum lycopersicum

Additional information

PIF proteins are not that stable, therefore special precautions should be taken during extraction and whole procedure should be performed in as little light as possible (light green light). Extraction of PIF proteins is described in Shen et al. (2007).,For detection of PIF5 please use the most sensitive detection reagent

Description

PIF5 (Phytochrome interacting factor 5) is a transcription factor negatively involved in the photochrome B signaling pathway, modulating PHYB at a posttranscriptional level. Replacement protein names :AtbHLH65, bHLH65, bHLH065, basic helix-loop-helix protein 65, photochromin interaction factor 1221123-like 6, PIL6, photochromin interaction factor like 6, transcription factor en103.

For research use only, not for clinical use.