Please, do not re-use PIF3 antibody solution after first incubation with your membrane as most of antibody will bind in this step and next result will not be reproducable,PIF3 protein runs at higher MW than expected, as observed previously (Al-Sady et al. 2006). PIF proteins are not that stable, therefore special precautions should be taken during extraction and whole procedure should be performed in as little light as possible (light green light).
PIF3(photochromin interaction Factor 3) is a transcription factor that plays an active role in the photochromin signaling pathway. It activates transcription by binding to the G-box. The subcell is located as the nucleus. Optional names: Basic spiral-loop-spiral protein 8,AtbHLH8, bHLH8, photochrome-associated protein 3, photochromin interaction factor 3, transcription factor EN100, EN100, bHLH transcription factor bHLH008, PAP3, photochromin associated protein 3,POC1, Photocurrent 1,ABI1, ABA insensitive 1,AtABI1.
For research use only, not for clinical use.