PIF3 | Phytochrome interacting factor 3 (goat antibody)
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PIF3 | Phytochrome interacting factor 3 (goat antibody)

Cat: PA01355
Size: 50 µg
Host: Goat
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide, derived from Arabidopsis thaliana PIF3, UniProt: O80536, TAIR: AT1G09530
Host
Goat
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
57 | ca, 65 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Cardamine hirsuta
Not reactive in
Nicotiana attenuata

Additional information

Please, do not re-use PIF3 antibody solution after first incubation with your membrane as most of antibody will bind in this step and next result will not be reproducable,PIF3 protein runs at higher MW than expected, as observed previously (Al-Sady et al. 2006). PIF proteins are not that stable, therefore special precautions should be taken during extraction and whole procedure should be performed in as little light as possible (light green light).

Description

PIF3(photochromin interaction Factor 3) is a transcription factor that plays an active role in the photochromin signaling pathway. It activates transcription by binding to the G-box. The subcell is located as the nucleus. Optional names: Basic spiral-loop-spiral protein 8,AtbHLH8, bHLH8, photochrome-associated protein 3, photochromin interaction factor 3, transcription factor EN100, EN100, bHLH transcription factor bHLH008, PAP3, photochromin associated protein 3,POC1, Photocurrent 1,ABI1, ABA insensitive 1,AtABI1.

For research use only, not for clinical use.