PhyA | Phytochrome A
  1. Home
  2. Products
  3. Plant Antibodies
  4. Plant Function Related Antibodies
  5. Plant Developmental Biology
Products
Online Inquiry

PhyA | Phytochrome A

Inquiry
Cat: PA00221
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Arachis hypogaea L., Nicotiana benthamiana, Nicotiana tabacum
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from conserved plant PhyA protein sequences including Arabidopsis  thaliana UniProt:P14712, TAIR:At1g59070; peptide sequence is not present in other plant phytochrome forms (B-E)
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
124 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Arachis hypogaea L., Nicotiana benthamiana, Nicotiana tabacum
Predicted reactivity
Brassica rapa, Cardamine hirsuta, Dacucus carota, Lathyrus sativus, Fragaria ananassa, Glycine max, Gossyoium hirsutum, Hordeum vulgare, Lotus corniculatus, Medicago truncatula, Nicotiana benthamiana (PhyA1), Nicotiana tabacum, Pisum sativum, Populus balsamifera, Ricinus communis, Solanum lycopersicum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

In vivo pull down assay for PhyA and western blot analysis of eluted proteins is described in Paik et al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340.,Careful sample collection is adviced to assure the best results with this antibody

Description

Phytochrome A (PhyA) is the main photoreceptor that mediates various plant responses to far-red (FR) light. Alternative protein names: far red long hypocotyl 2, far red long hypocotyl 1, far red long hypocotyl 8.

For research use only, not for clinical use.