Photosynthesis Tool Kit - quantitation

Product information - Primary antibodies: Product number: Product name: Reconstitution: Recommended dilution: AS03 037 Rabbit Anti-RbcL Global antibody For reconstitution see lable on respective tube. 1:5000-10 000 with ECL AS10 939 Rabbit Anti-PsaC Global antibody For reconstitution see lable on respective tube. 1:1000 with ECL AS05 084 Rabbit Anti-PsbA Global antibody For reconstitution see lable on respective tube. 1:10 000 with ECL * All primary antibodies in this kit are raised in rabbits.Product information - Protein standards: Product number: Product name: Concentration: Size: Western Blot – Positive Control: AS01 016S PsbA * 0.25 pmol/ μl 41.5 kDa# To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control. AS01 017S RbcL * 0.15 pmol/ μl 52.7 kDa To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a RbcL signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control. AS04 042S PsaC * 0.15 pmol/μl 11.5 kDa# To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 µl). For most applications a sample load of 0.2 µg of chlorophyll will give a PsaC signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems as a positive control. *These proteins are larger than a respective native protein due to the addition of His-tag* For reconstitution of standards see lable on respective tube.Product information - Secondary antibody:AS09 602-trial Goat anti-Rabbit IgG (H&L), HRP conjugated, 20 µl (2x10 µl)Product information - ECLreagent:AS16 ECL-N-10 AgriseraECL Bright (10 ml trial pack) Educational information about Quantitative western blot can be found here:  detailed method description, video tutorial,Estimation of PSI to PSII ratio can be done using quantitative western blot technique using anti-PsaC (PSI) and PsbA (PSII) antibodies. References: Brown et al. (2007). Resource dynamics during infection of Micromonas pusilla by virus MpV-SP1. Environmental Microbiology 9(11): 2720-2727. Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.