Phosphothreonine

Cat: PA01253

Size: 400 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.

Datasheet:

Product Info

Immunogen

KLH-conjugated phosphothreonine

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified in PBS, 50% glycerol, 0.01% sodium azide.

Format

Liquid

Storage

Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0,01 % sodium azide, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)

Recommended dilution

ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)

Reactivity

Confirmed reactivity

Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Affinity purified in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.25 mg/ml. This antibody is recognizing proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phosphotyrosine.,2 µg/ml of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions.

Description

Phosphothreonine is a phosphorylated ester of phosphorous amino acid and threonine.

For research use only, not for clinical use.