Products
Online Inquiry
Phosphomethylpyrimidine synthase, chloroplastic (THIC), Recombinant Protein
Cat: RP01661
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Phosphomethylpyrimidine synthase, chloroplastic (THIC) , partial
Product Gene Name
THIC recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
71,987 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Phosphomethylpyrimidine synthase
NCBI/Uniprot Data
NCBI Accession #
NP_001189634.1
NCBI GI #
334184567
NCBI GenBank Nucleotide #
NM_001202705.1
NCBI GeneID
817513
NCBI Official Full Name
thiaminC
NCBI Official Symbol
THIC
NCBI Official Synonym Symbols
PY; PYRIMIDINE REQUIRING; T27A16.27; T27A16_27; thiaminC
NCBI Protein Information
thiaminC
NCBI Summary
Encodes a protein involved in thiamin biosynthesis. The protein is an iron-sulfur cluster protein predicted to catalyze the conversion of 5-aminoimidazole ribonucleotide (AIR) to hydroxymethylpyrimidine (HMP) or hydroxymethylpyrimidine phosphate (HMP-P). A severe reduction of THIC levels in plants decreases vitamin B1 (thiamin diphosphate (TPP)) levels and also leads to changes in the levels of numerous other metabolites since so many primary metabolic enzymes require a TPP co-factor. thiC mutants are chlorotic and arrest in their development at the cotyledon stage. A N-terminal targeting sequence directs the THIC protein to the chloroplast stroma. A conserved TPP-binding site is located in the 3' UTR of the At2g29630.2 gene model, and is predicted to function as a riboswitch. The riboswitch controls the formation of transcripts with alternative 3' UTR lengths, which affect mRNA accumulation and protein production. THIC transcripts are observed in seedlings 5 or more days after germination, and light promotes the expression of this gene. Recessive mutant isolated by Redei. Leaves but not cotyledons white, lethal; restored to normal by thiamine or 2,5-dimethyl-4-aminopyrimidine.
UniProt Gene Name
THIC
UniProt Synonym Gene Names
PY; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Protein THIAMINE C
UniProt Protein Name
Phosphomethylpyrimidine synthase, chloroplastic
UniProt Synonym Protein Names
Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Protein PYRIMIDINE REQUIRING; Thiamine biosynthesis protein ThiC; Protein THIAMINE C
UniProt Primary Accession #
O82392
UniProt Related Accession #
O82392
UniProt Comments
Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.
For research use only, not for clinical use.