Phospholipase A1-IIgamma (DSEL), Recombinant Protein
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Phospholipase A1-IIgamma (DSEL), Recombinant Protein

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Cat: RP01252
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Phospholipase A1-IIgamma (DSEL)
Product Gene Name
DSEL recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MKRKKKEEEE EKLIVTREFA KRWRDLSGQN HWKGMLQPLD QDLREYIIHY GEMAQAGYDT FNINTESQFA GASIYSRKDF FAKVGLEIAH PYTKYKVTKF IYATSDIHVP ESFLLFPISR EGWSKESNWM GYVAVTDDQG TALLGRRDIV VSWRGSVQPL EWVEDFEFGL VNAIKIFGER NDQVQIHQGW YSIYMSQDER SPFTKTNARD QVLREVGRLL EKYKDEEVSI TICGHSLGAA LATLSATDIV ANGYNRPKSR PDKSCPVTAF VFASPRVGDS DFRKLFSGLE DIRVLRTRNL PDVIPIYPPI GYSEVGDEFP IDTRKSPYMK SPGNLATFHC LEGYLHGVAG TQGTNKADLF RLDVERAIGL VNKSVDGLKD ECMVPGKWRV LKNKGMAQQD DGSWELVDHE IDDNEDLDF
Sequence Positions
1-419, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
47,792 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Phospholipase

NCBI/Uniprot Data

NCBI Accession #
NP_193590.1
NCBI GI #
15233922
NCBI GenBank Nucleotide #
NM_117969.3
NCBI GeneID
827587
NCBI Official Full Name
alpha/beta-Hydrolases superfamily protein
NCBI Official Symbol
DSEL
NCBI Official Synonym Symbols
Arabidopsis thaliana DAD1-like seeding establishment-related lipase; AtDSEL; DAD1-like seeding establishment-related lipase; F28J12.210; F28J12_210
NCBI Protein Information
alpha/beta-Hydrolases superfamily protein
NCBI Summary
DSEL is cytosolic acylhydrolase that shows prefential lipase activity against the sn-1 position of several classes of lipids, including 1,3-diacylglycerols and 1-monoacylglycerols. Overexpression of DSEL leads to increased peroxisome and oil body levels in cotyledons and reduced beta-oxidation activity in seedlings.
UniProt Gene Name
DSEL
UniProt Synonym Gene Names
AtDSEL; Phospholipase DSEL
UniProt Protein Name
Phospholipase A1-IIgamma
UniProt Synonym Protein Names
DAD1-like seedling establishment-related lipase; AtDSEL; Phospholipase DSEL
UniProt Primary Accession #
O49523
UniProt Secondary Accession #
Q8GXS9
UniProt Related Accession #
O49523
UniProt Comments
Acylhydrolase that catalyzes the hydrolysis of 1,3-diacylglycerol (1,3-DAG) and 1-monoacylglycerol (1-MAG) at the sn-1 position. High activity toward 1,3-DAG and 1-MAG, but low activity toward 1,2-diacylglycerol (1,2-DAG) and 1-lysophosphatidylcholine (1-LPC), and no activity toward phosphatidylcholine (PC), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), triacylglycerol (TAG) and 2-monoacylglycerol (2-MAG). May be involved in the negative regulation of seedling establishment by inhibiting the breakdown, beta-oxidation and mobilization of seed storage oils.

For research use only, not for clinical use.