Phaseolus Vulgaris Erythroagglutinin (PHA-E), Fluorescein
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Phaseolus Vulgaris Erythroagglutinin (PHA-E), Fluorescein

Cat: PL00680
Size: 2 mg
Sugar Specificity: Galactose, Complex Structures
Datasheet:

Description

Phasebean lectins are the name of a family of lectins, each consisting of four subunits. There are two different types of subunits. One of these appears to be primarily involved in red blood cell agglutination and is designated as the "E" subunit (red agglutinin). Another type is involved in lymphocyte agglutination and mitotic activity and is known as the "L" subunit (leukocyte lectin). These subunits combine to produce five isolates. PHA-E has strong hemagglutination activity, but is a poor mitogen. Fluorescein-labeled PHA-E has the appropriate amount of fluorescent dye binding to provide optimal staining properties for this lectin. This conjugated substance basically does not contain unconjugated fluorescent dyes. The excitation maximum is 495nm and the emission maximum is 515nm.

Product Info

Conjugate
Fluorescein
Concentration
2 mg active conjugate/ml
Sugar Specificity
Galactose, Complex Structures
Storage
2-8℃
Excitation
495-500 nm
Emission
514-521 nm
Fluorescence
Green
Application
Immunofluorescence, Glycobiology
Recommended dilution
The recommended concentration range for use is 5-20 µg/ml.
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 5mg/ml Beta-Cyclodextrin

For research use only, not for clinical use.