Lima bean LBA is isolated from Lima bean seeds and purified by affinity chromatography. LBA consists of 30,000 Da subunits, two of which are connected by disulfide bonds. Each subunit contains cysteine residues, which are necessary for its carbohydrate and metal ion binding activity. It has carbohydrate specificity of terminal GalNAcI ±(1,3)Gal and is eluted with n-acetylgalactosamine. LBA agglutinated type A erythrocytes. The crosslinked 6B SeparoporeA ® is properly coupled to the Phaseolus limensis lectin (LBA), providing a fast-flowing cartridge that can capture the affinity-bound material and retain it during washing, and then elute the affinity-purified material.
For research use only, not for clinical use.