Modifications to backbone
gentamycin-resistant marker was removed and kanamycin-resistant marker (NOS promoter:NPTII:NOS terminator) was inserted.
Selectable markers
Kanamycin
Bacterial Resistance(s)
Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
Growth Strain(s)
ccdB Survival