PGL35 | Plastoglobulin 35; FIB1a; FBN1a

Cellular [compartment marker] of chloroplast plastoglobules.For IC samples are embedded in Lowicryl HM20 and sectioned into 100-nm-thick sections and placed on Formvar-coated gold slot grids. The sections are blocked for 20 min with a 5% (w/v) solution of nonfat milk in TBS plus 0.1%Tween 20 (TBST). Anti-PGL antibodies are diluted 1:20 in a solution of 2.5% nonfat milk in TBST at room temperature for 1 h. The sections are rinsed in a stream of TBS plus 0.5% Tween 20 and then transferred to the secondary antibody (anti-rabbit IgG 1:20 in TBST) conjugated to 10-nm gold particles for 1 h. images of localization can be found in Austin et al. (2006).,AtPGL35 is highly similar to Pisum sativum PG1

Plastoglobinin is a lipoprotein particle found in chloroplasts. They are thought to have a lipid storage function. Recent data suggest that plastoglobinin can also be metabolically active, involved in tocopherol synthesis and possibly other pathways. Immunogen: alias AtPap1, fibrinogen 1, possibly plastid lipoid-associated protein 1.