Immunogen
Recombinant, soluble N-terminal domain of Arabidopsis thaliana Pex14p UniProt:Q9FE40, TAIR: At5g62810) that mediates PEX5 and PEX19 binding. The transmembrane and coiled-coil domains of PEX14 are replaced with a dual StrepII-His6 tag.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution
For reconstitution add 25 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 10 000 (WB)
Expected | apparent MW
55,5 | 75-65 kDa (depending on the gel system)