Shipping
Extracted proteins are vialed at 5 mg/mL and shipped on dry ice. Proteins are also available in Denaturing SDS Sample Buffer for Western blotting only.
Additional information
Protein quality can be examined by denaturing SDS-PAGE analysis, protein band clarity and resolution using Coomassie Blue staining. Protein integrity was examined by immunoblotting using a specific beta-actin antibody.
Application
The total protein can be immediately used for Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gel and SDS capillary electrophoresis, enzyme activity analysis, gel transfer test, protein protein interaction and tissue specific expression.
Description
Pepper total protein (protein lysate) is isolated from freshly harvested tissues of single healthy normal donor. Tissues are homogenized in protein lysis buffer supplemented with a cocktail of protease inhibitors to minimize proteolysis and precisely quantified.