PEPC | Phosphoenolpyruvate carboxylase positive control/quantitation standard

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Cat: PA01820

Size: 100 µl

Datasheet:

Product Info

Format

Lyophilized in glycerol.

Reconstitution

For reconstitution add 60 µl of steril water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2 μg of chlorophyll/well will give a RbcL signal in this range. Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Expected | apparent MW

110 | 105 kDa

Reactivity

Additional information

The PEPC protein standard can be used in a combination with Agrisera global PEPC antibiody to quantitate PEPC from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Description

Phosphoenolpyruvate carboxylase; EC 4.1.1.31) is an important regulator of photosynthetic carbon metabolism in C4 and CAM plants. This is the first enzyme in this pathway, the PEPC enzyme, which is encoded by a small polygenic family. Several subtypes of PEPC have been found in corn, sorghum and sugar cane. These isomers are involved in a variety of functions such as initial fixation of atmospheric CO2 (= C4 PEPC) and outcross functions associated with nitrogen assimilation or amino acid biosynthesis (Lepiniec et al. 1994).

For research use only, not for clinical use.