P-Tyr | Phosphotyrosine (clone G104)

Cat: PA01874

Size: 100 µg

Host: Mouse

Clonality: Monoclonal

Confirmed reactivity: Antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated, Does not cross react with phosphoserine or phosphothreonine

Datasheet:

Product Info

Immunogen

Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3’-dimentrylaminopropyl) carbodiimide

Host

Mouse

Clonality

Monoclonal

Purity

Total IgG.

Format

Liquid

Storage

Store at -20°C for one year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Reactivity

Confirmed reactivity

Antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated, Does not cross react with phosphoserine or phosphothreonine

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Protein G purified IgG1 in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml,1 µg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis

Description

Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzyme activity. Phosphotyrosine antibodies are helpful for the identification of tyrosine kinase substrates.

For research use only, not for clinical use.