Shipping
Extracted proteins are vialed at 5 mg/mL and shipped on dry ice. Proteins are also available in Denaturing SDS Sample Buffer for Western blotting only.
Additional information
Quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE using Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.
Application
Total protein is immediately available for Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gel and SDS - capillary electrophoresis, enzyme activity assays, gel shift assays, protein-protein interactions and tissue-specific expression.
Description
Orange total protein (protein lysate) is isolated from freshly harvested tissues of single healthy normal donor. Tissues are homogenized in protein lysis buffer supplemented with a cocktail of protease inhibitors to minimize proteolysis and precisely quantified.