NtrC | Thioredoxin reductase (TR/TRxR) (higher plants)

Inquiry

Cat: PA00831

Size: 200 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7 The peptide is perfectly conserved in Arabidopsis thaliana NTRC, UniProt: , TAIR: AT2G41680

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 300 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 500 (WB)

Expected | apparent MW

55.3 kDa (Chlamydomonas reinhardtii), 57.9 kDa (Arabidopsis thaliana), further processed into the matured form

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Anthurium amnicola, Brachypodium distachyon, Brassica napus, Brassica oleracea, Capsella rubella, Capsicum annuum, Cephalotus follicularis, Chlamydomonas reinhardtii, Chlamydia sp., Cicer arietinum, Citrus sinensis, Cucumis melo, Cucumis sativus, Dendrobium catenatum, Eutrema salsugineum, Genlisea aurea, Ostreococcus luminarius, Synechococcus sp., Glycine soja, Glycine max, Gossypium raimondii, Hordeum vulgare, Juglans regia, Leersia perrieri, Manihot esculenta, Medicago truncatula, Musa acuminata subsp. malaccensis, Nicotiana tabacum, Nelumbo nucifera, Noccaea caerulescens, Oryza barthii, Phoenix dactylifera, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Zea mays, Vigna angularis var. angularis, Vitis vinifera

Additional information

Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria

Description

Thioredoxin Reductase (TR, TrxR) is the only known enzyme that reduces thioredoxin (Trx) (EC 1.8.1.9) and is involved in the protection of chloroplasts against oxidative damage. Dodoxin activity is essential for cell growth and survival. The NtrC protein in Arabidopsis appears to have an isomer, including the n-terminal reductase domain and the C-terminal domain associated with thioredoxin. The NtrC proteins of Arabidopsis and Chlamydomonas Rhine are about 568 amino acids in length, but contain about 80 amino acid signaling peptides, and the mature protein size is about 488 amino acids.

For research use only, not for clinical use.