Microsomal fraction is recommended to be used as it will have higher amount of NRT2,1 protein, If a total protein extract is used SDS should be applied from the beginning in extraction buffer as well as protease inhibitor coctail
NRT2.1 is a nitrate transporter with high affinity and can be upregulated by nitrate. Acts as an inhibitor of lateral root formation independent of nitrate absorption. Synonyms: Protein ACH1, lateral root initiation 1,ACH1, ATNRT2.1, ATNRT2:1, LIN1, nitrate transporter 2, nitrate transporter 2.1, nitrate transporter 2:1,NRT2, NRT2.1, nrT2:1,NRT2, 1AT
For research use only, not for clinical use.