For successful detection using NPR1 antibody please follow protocol suggested below. NPR1 protein readily oligomerizes, in addition to any naturally occurring oligomer, during extraction. Therefore 50 mM DTT has to be used as well as denaturation at 75°C for 15 minutes. Engogenous NPR1 level is very low, thereofre SA treatment is absolutely necessary for good detection.This antibody is recognizing NPR1-GFP in the 35S overexpression line.,Please note that depending upon detection system you are using, longer exposure time may be required with this antibody
regulatory protein NPR1 (NPR1) is a key positive regulator of the SA-dependent signaling pathway and negatively regulates the ja dependent signaling pathway. Control the incidence of systemic acquired drug resistance (SAR). Subcellular localization: cytoplasm, nucleus (after salicylic acid induction or after pathogen infection). Aliases: Containing BTB/POZ domain protein NPR1, non-induced immune protein 1,Nim1, salicylic acid insensitive protein 1,Sai1, ATNPR1.
For research use only, not for clinical use.