NPR1 | Nonexpresser of PR genes 1 (0,5 mg)
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NPR1 | Nonexpresser of PR genes 1 (0,5 mg)

Cat: PA01607
Size: 0,5 mg (10x 50 µg)
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide, chosen from NPR1 sequence of Arabidopsis thaliana, TAIR: AT1G64280, UniProt: P93002
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water to each tube.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
66 | 66 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis thaliana
Not reactive in
Nicotiana benthamiana, Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum

Additional information

For successful detection using NPR1 antibody please follow protocol suggested below. NPR1 protein readily oligomerizes, in addition to any naturally occurring oligomer, during extraction. Therefore 50 mM DTT has to be used as well as denaturation at 75°C for 15 minutes. Engogenous NPR1 level is very low, thereofre SA treatment is absolutely necessary for good detection.This antibody is recognizing NPR1-GFP in the 35S overexpression line.,Please note that depending upon detection system you are using, longer exposure time may be required with this antibody

Description

regulatory protein NPR1 (NPR1) is a key positive regulator of the SA-dependent signaling pathway and negatively regulates the ja dependent signaling pathway. Control the incidence of systemic acquired drug resistance (SAR). Subcellular localization: cytoplasm, nucleus (after salicylic acid induction or after pathogen infection). Aliases: Containing BTB/POZ domain protein NPR1, non-induced immune protein 1,Nim1, salicylic acid insensitive protein 1,Sai1, ATNPR1.

For research use only, not for clinical use.