Nitrotyrosine (mouse monoclonal) (clone EM-30)

Inquiry

Cat: PA01529

Size: 0,1 mg

Host: Mouse

Clonality: Monoclonal

Confirmed reactivity: Nitrotyrosine Nitrotyrosine

Datasheet:

Product Info

Immunogen

NO2-Tyr-CH2-Thyroglobulin

Host

Mouse

Clonality

Monoclonal

Purity

Immunoglobulin purified on Protein A.

Format

Liquid at 1 mg/ml.

Storage

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)

Recommended dilution

(IHC) paraffin: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia. (IHC) frozen sections: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia. (WB): positive control: NO2-BSA, NO2-HSA.

Reactivity

Confirmed reactivity

Nitrotyrosine Nitrotyrosine

Additional information

This antibody detects nitrotyrosine group in biological material, It can be used as a marker for peroxynitrite formation in particular tissues

Description

Nitrotyrosine can be detected in proteins in a variety of tissues and is often associated with pathological conditions. Nitric oxide reacts with superoxide to form peroxynitrite, which can undergo heterocleavage to form nitrogen and hydroxyl ions. The nitration of tyrosine residues by nitro ions forms nitrotyrosine groups in the corresponding protein. Therefore, nitrotyrosine is a marker of inflammation-related tissue damage.

For research use only, not for clinical use.