Nitrilase 1 (NIT1), Recombinant Protein
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Nitrilase 1 (NIT1), Recombinant Protein

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Cat: RP01012
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Nitrilase 1 (NIT1)
Product Gene Name
NIT1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MSSTKDMSTV QNATPFNGVA PSTTVRVTIV QSSTVYNDTP ATIDKAEKYI VEAASKGAEL VLFPEGFIGG YPRGFRFGLA VGVHNEEGRD EFRKYHASAI HVPGPEVARL ADVARKNHVY LVMGAIEKEG YTLYCTVLFF SPQGQFLGKH RKLMPTSLER CIWGQGDGST IPVYDTPIGK LGAAICWENR MPLYRTALYA KGIELYCAPT ADGSKEWQSS MLHIAIEGGC FVLSACQFCQ RKHFPDHPDY LFTDWYDDKE HDSIVSQGGS VIISPLGQVL AGPNFESEGL VTADIDLGDI ARAKLYFDSV GHYSRPDVLH LTVNEHPRKS VTFVTKVEKA EDDSNK
Sequence Positions
1-346, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
37,502 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Nitrilase

NCBI/Uniprot Data

NCBI Accession #
NP_001078234.1
NCBI GI #
145332737
NCBI GenBank Nucleotide #
NM_001084765.1
NCBI GeneID
823556
NCBI Official Full Name
nitrilase 1
NCBI Official Symbol
NIT1
NCBI Official Synonym Symbols
A. THALIANA NITRILASE 1; ATNIT1; NITI; nitrilase 1; NITRILE AMINOHYDROLASE
NCBI Protein Information
nitrilase 1
NCBI Summary
Mutants are resistant to indole-3-acetonitrile (IAN). NIT1 catalyzes the terminal activation step in indole-acetic acid biosynthesis. Predominantly expressed isoform of nitrilase isoenzyme family. Aggregation of NIT1 in cells directly abutting wound sites is one of the earliest events associated with wound and herbicide-induced cell death. The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide. It is also involved in the conversion of IAN to IAM (indole-3-acetamide) and other non-auxin-related metabolic processes.
UniProt Gene Name
NIT1
UniProt Protein Name
Nitrilase 1
UniProt Primary Accession #
P32961
UniProt Secondary Accession #
O04908; Q42543; Q53YI1; Q94B53
UniProt Related Accession #
P32961
UniProt Comments
Can convert indole-3-acetonitrile to the plant hormone indole-3-acetic acid.

For research use only, not for clinical use.