NEDD8-activating enzyme E1 regulatory subunit (AXR1), Recombinant Protein
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NEDD8-activating enzyme E1 regulatory subunit (AXR1), Recombinant Protein

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Cat: RP02002
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 regulatory subunit (AXR1) , partial
Product Gene Name
AXR1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
60,035 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
NEDD8-activating enzyme E1 regulatory

NCBI/Uniprot Data

NCBI Accession #
NP_172010.1
NCBI GI #
15220442
NCBI GenBank Nucleotide #
NM_100396.4
NCBI GeneID
839286
NCBI Official Full Name
NAD(P)-binding Rossmann-fold superfamily protein
NCBI Official Symbol
AXR1
NCBI Official Synonym Symbols
AUXIN RESISTANT 1; AUXIN RESISTANT PROTEIN 1; YUP8H12.21; YUP8H12_21
NCBI Protein Information
NAD(P)-binding Rossmann-fold superfamily protein
NCBI Summary
Encodes a subunit of the RUB1 activating enzyme that regulates the protein degradation activity of Skp1-Cullin-Fbox complexes, primarily, but not exclusively, affecting auxin responses. Acts alongside AS1 to exclude BP expression from leaves.
UniProt Gene Name
AXR1
UniProt Protein Name
NEDD8-activating enzyme E1 regulatory subunit AXR1
UniProt Synonym Protein Names
Auxin-resistance protein AXR1
UniProt Primary Accession #
P42744
UniProt Related Accession #
P42744
UniProt Comments
Regulatory subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1. Plays an important role in auxin response (PubMed:8321287). Regulates the chromosomal localization of meiotic recombination by crossovers (COs) and subsequent synapsis, probably through the activation of a CRL4 complex (PubMed:25116939). Required for E3-mediated protein degradation in response to auxin, jasmonic acid and cold stress. Required for the COP1-COP10-CSN-mediated repression of photomorphogenesis in the dark (PubMed:12368504). May function redundantly with AXL1 in the RUB conjugating pathway (PubMed:17655650). Seems not to be functionally equivalent to AXL1 in vivo (PubMed:21311953).

For research use only, not for clinical use.