NdbA | Thylakoid Localized Type 2 NAD(P)H Dehydrogenase

Inquiry

Cat: PA01719

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Synechocystis sp. PCC 68

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from NdbA protein sequence of Synechocystis sp. PCC 6803, UniProt:P73739 Chosen peptide is not conserved in NdbB and NdbC.

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 2000 (WB)

Expected | apparent MW

49 | 46 kDa

Reactivity

Confirmed reactivity

Synechocystis sp. PCC 68

Predicted reactivity

Bacillus subtilis

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Protein extraction: harvested cells are suspended in a buffer containing 50 mM Hepes-NaOH, pH 7,5, 30 mM CaCl2, 800 mM sorbitol, 1 mM ε-amino-n-caproic acid, and the cells are broken by vortexing 6×1 min at 4°C in the presence of glass beads, Protein samples are solubilized in Laemmli buffer containing 6 M urea and separated on a gel in presence of 6M urea

Description

NdbA(thylakoid localization type 2 NAD(P)H dehydrogenase) is localized to the thylakoid membrane and is expressed at a low level in the wild type under photoautotrophic conditions. NdbA is heavily induced under photoactivated heterotrophic growth conditions.

For research use only, not for clinical use.